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Abstracts of Evaluation Studies Clinton H. Howard and Kimberly Lloyd Royal BodyCare is conducting an ongoing series of laboratory and clinical studies designed to evaluate the nutritional characteristics and benefits of Microhydrin® (250 mg per cap) as a mineral antioxidant. The following is a brief summary of the results of studies completed as of July 30, 1999. Microhydrin® in Intra and Extra Cellular Hydration A double blind placebo controlled pilot study was conducted using the RJL Bioelectrical Impedance Analyzer developed by R. J. Liedtke. Eight subjects received 4 capsules of Microhydrin per day for two weeks and were crossed over receiving 4 capsules per day of a placebo (rice bran flour). Averaged values showed increase intra cellular and extracellular hydration due to the consumption of Microhydrin as compared to the placebo group. Body Cell Mass (BCM) and Intracellular Water (ICW) are assessments of intracellular volume and water inside the cell respectively. Extracellular Body Water, water that bathes the cells, also showed increased volume. Intracellular water as an indicator of cell integrity is found to be higher in babies but decreases as adults age or lose body cell mass. Healthy tissue cells hold water within the cells and have higher anabolic (energy producing) function rather than catabolic (breaking down) function.
A controlled clinical trial showed that in eight normal adult subjects, seven of the nine Biological Terrain Assessment parameters improved. Saliva pH, saliva rH2, blood resistivity, and urine resistivity showed statistically significant improvement (p >0.05) for these eight subjects after 18 days of supplementation with 4 Microhydrin per day on a schedule of one in the morning, two at midday and one in the evening. Three other parameters (urine pH, urine rH2, and saliva resistivity) also showed improvement over the 18-day supplementation period when their averaged changes were calculated. Blood pH and blood rH2 were the only parameters that did not change during the supplementation period. These values may not change as readily due to the heterogenous and stable nature of blood. The nutritionists and physicians who monitored the study with Biological Terrain Assessment results and other clinical data observed no adverse side effects in this group during the supplementation period. Presented at the American College of Nutrition Symposium on Advances in Clinical Nutrition. Albuquerque, New Mexico, October, 1998. Microhydrin® Protection Against Oxidative Stress A double blind placebo controlled crossover pilot study was conducted on 7 patients who received 4 caps/day of Microhydrin for two weeks, and received 4 caps/day of a placebo for two weeks. Urine alkenal/creatinine ratios were measured. During Microhydrin supplementation, a 43% increase in free radical protection was observed as compared to the placebo group. Microhydrin was observed in this study to protect against serum alkenals. Alkenals are the oxidative products of serum lipid peroxides occurring from free radical attacks on cellular lipid membranes and lipoproteins. They are indicators of free radical damage in the body associated with a higher risk of age related diseases.
Microhydrin®
Increased Mitochondria Membrane Confocal fluorescence microscopy revealed that when cells were incubated with Microhydrin at a concentration of 200 ug/ml, approximately 10% of the mitochondria showed enhanced membrane potential. Normal cells show a membrane potential of 120-140 mV. However, after addition of Microhydrin to the cell culture media, 10% of the total mitochondria within the individual cells measured greater than 140 mV. This test measures changes in the electronic potential across the mitochondria membrane due to enhanced electron activity. These investigators are continuing to study the properties of Microhydrin towards enhancement of NADH and ATP production in various assays with fluorescent microscopy. Evaluation of Microhydrin® in the Production of NADH ATP production, the principal source of energy in the mitochondria, depends on the production of NADH. Reduced hydrogen, generated by the Kreb’s Cycle, is carried by NADH into the mitochondria for the electron transport chain reactions that will ultimately create a molecule of water and ATP. Hydrogen is one of the most important elements donating an electron, an electron pair or its proton to reduction/oxidation reactions of numerous enzymes and intermediates within the cell’s metabolic pathways. In vivo assays measured the direct conversion of NAD+ to NADH by the addition of Microhydrin. The sequential addition of increasing concentrations of Microhydrin to NAD+ showed a linear increase in NADH production as measured by its absorption at 350 nm. Copyright© 1999 Royal BodyCare, Inc. All Rights Reserved. |
