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Super Oxide Scavenging Assays With Microhydrin
The superoxide mediated reduction of cytochrome c showed that the reduced silicate mineral inhibited the superoxide-mediated reduction of cytochrome c, indicating that the reduced silicate is reducing, or scavenging, the superoxide radical also:
Mic (H:–) + 02– + H+ à Mic + H202
In this assay Microhydrin was found to inhibit and prevent the reduction of cytochrome c by superoxide:
02–+ Cyt c(Fe+ 3) à 02 + Cyt c(Fe+2)
An alternative assay to further elucidate the activity of the reduced silicate mineral, observed the oxidation of epinephrine to adrenochrome by superoxide (unpublished data).
Microhydrin
02– + epinephrine à H202 + adrenochrome
Superoxide Hydrogen peroxide
free radical
 Data is shown in Table 1 for superoxide dismutase-like (SOD) activity based on the oxidation of epinephrine by superoxide.
In this assay the reduced hydrogen mineral silicate (Microhydrin) scavenged the superoxide ion again. Quantitatively, half-maximal inhibition was achieved at a Microhydrin concentration of 90
µg/ml (Table 1). When concentrations of Microhydrin were at 60 µg/ml or greater, epinephrine oxidation was inhibited showing that the silicate mineral reduced the superoxide free radical. The assay conditions utilized superoxide dismutase at a concentration of 50µg/ml with a conversion rate of 0.15 µg/ml (unpublished data) (Table 1, Figure 2).
References
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